Determination of Diazinon in Fruits and Vegetables by ELISA

Zhihua Fan and Rodney J. Bushway*

Department of Food Science and Human Nutrition, 5736 Holmes Hall, University of Maine, Orono, ME 04469-5736, USA

Article history:
Received June 26, 1997
Accepted October 14, 1997

Key words:
ELISA, diazinon, fruits, vegetables, GC-AED

Summary:
A commercial polyclonal plate enzyme-linked immunosorbent assay (ELISA) was applied to the analysis of CliaZiTi011 in fruits and vegetables. The produce was extracted by polytroning 10 g in 20 mL of methanol for 3 min followed by a clean-up with C18 Sep-Paks_ All samples and standards were run in 10% methanol. Repro­ducibility studies yielded coefficients of variation (CV) from 2.0 to 14%. Fruits and vegetables spiked at five different levels had percent recoveries ranging from 80 to 95 (CV of 2.2-15%) Comparative analysis of 59 positive diazinon samples by enzyme-linked lonniunosorbent assay and the gas chromatography - atomic emission detector method showed good agreement with a correlation coefficient of 0.987.