Measurement of Yeast Viability/Mortality in the Presence of Chromium(VI)

Peter Raspor¹,*, Martin Batič^1,2 and Polona Jamnik<sup>1

1</sup>Food Science and Technology Department, Biotechnical Faculty, Department of Biotechnology, University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia
²Biotechnology Department, Mlinotest d. d., Tovarniška 14, 5270 Ajdovščina, Slovenia

Article history:
Received October 29, 1998
Accepted February 15, 1999

Key words:
yeasts, cell viability, mortality, Cr(VI) toxicity

Summary:
The aim of this work was to develop a mathematical model – a set of equations for studying the effect of Cr(VI) on yeast viability/mortality. The model was built with parameters and a two-stream approach has been developed which includes four parameters that indicate viability like population condition (PC), cell viability in the buffer (V_B), cell viability in the buffer supplemented with the effector (V_EB), specific cell viability (V_S), and three parameters that indicate mortality as follows: cell mortality in the buffer (M_B), cell mortality in the buffer supplemented with the effector (M_EB) and specific cell mortality (M_S). The parameters were calculated on the basis of primary results
the total cell number per mL (N_T) and the number of viable cells per mL (N_CFU). The total cell number was obtained microscopically with a hemacytometer and yeast viability was determined with the colony count method (spread plate method).

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